Starring Seth Rogan and Zac Efron Bad Neighbours had no trouble de-throning the web slinger with an £8.5 million debut.
Spider-Man 2 falls into second place with £1.3 million bringing it's total gross to £22 million after 4 weeks.
Also new this week are:
Sabotage - Arnold Schwarzenegger's latest
La Cenerentola - the latest from the Met Opera
The Wind Rises
Before The Winter Chill
Last year saw the much anticipated Star Trek Into Darkness enter at the top knocking Iron Man 3 into second place.
Five years ago saw JJ Abrahams first Star Trek film enter the box office at the top knocking X-Men Origins: Wolverine into 3rd place with Coraline entering at 2.
Ten years ago had Van Helsing debuting at the top meaning Eternal Sunshine of The Spotless Mind fell to number 2.
Fifteen years ago had I Still Know What You Did Last Summer go to the top on it's first week leaving The Waterboy to have a single week at the top.