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Starring Seth Rogan and Zac Efron Bad Neighbours had no trouble de-throning the web slinger with an £8.5 million debut.
Spider-Man 2 falls into second place with £1.3 million bringing it's total gross to £22 million after 4 weeks.
Also new this week are:
Sabotage - Arnold Schwarzenegger's latest
La Cenerentola - the latest from the Met Opera
The Wind Rises
Before The Winter Chill
Last year saw the much anticipated Star Trek Into Darkness enter at the top knocking Iron Man 3 into second place.
Five years ago saw JJ Abrahams first Star Trek film enter the box office at the top knocking X-Men Origins: Wolverine into 3rd place with Coraline entering at 2.
Ten years ago had Van Helsing debuting at the top meaning Eternal Sunshine of The Spotless Mind fell to number 2.
Fifteen years ago had I Still Know What You Did Last Summer go to the top on it's first week leaving The Waterboy to have a single week at the top.
The follow up Spider-Man film added another £2 million over the weekend bringing it's total UK gross to just why of £ 20 million.
With The Other Woman staying put at 2 the highest new film was the Paul W.S. Anderson directed Pompeii which entered at 3. Based on the historical events surrounding the Italian village Pompeii took £1.2 million over the weekend.
Other new films this week are:
Blue Ruin (15)
This time last year there was still no moving Iron Man 3 as he spent another week at the top with the highest new film 21 and Over entering at 2.
Five years ago X-Men Origins: Wolverine debuted on the chart at the top leaving Hannah Montana The Movie to land at 2. State of Play fell from the top to 3.
Ten years ago Kill Bill Volume 2 was still riding the top slot keeping Eternal Sunshine of the Spotless Mind from debuting at number 1, it had to settle for 2.
Fifteen years ago The Waterboy starring Adam Sandler was the new film at the top of the box office, knocking 8MM down to second place.
Twenty years ago Four Wedding and a Funeral was the big film for May with The Naked Gun 33 1/3 The Final Insult and The Crow also doing well while twenty five years ago Warlock starring Julian Sands was the big film with Mississippi Burning and Beaches also doing well.